Terumo spectra optia leukapheresis of cynomolgus macaques for hematopoietic stem cell and T cell collection.
Helen L WuJustin M GreeneTonya SwansonChristine Shriver-MunschKimberly ArmantroutWhitney C WeberKatherine B BatemanNicholas M MaierMina NorthrupAlfred W LegasseCassandra MoatsMichael K AxthelmJeremy SmedleyRichard T MaziarzLauren Drew MartinTheodore HobbsBenjamin J BurwitzJonah B SachaPublished in: Journal of clinical apheresis (2020)
Macaques are physiologically relevant animal models of human immunology and infectious disease that have provided key insights and advanced clinical treatment in transplantation, vaccinology, and HIV/AIDS. However, the small size of macaques is a stumbling block for studies requiring large numbers of cells, such as hematopoietic stem cells (HSCs) for transplantation, antigen-specific lymphocytes for in-depth immunological analysis, and latently-infected CD4+ T-cells for HIV cure studies. Here, we provide a detailed protocol for collection of large numbers of HSCs and T-cells from cynomolgus macaques as small as 3 kg using the Terumo Spectra Optia apheresis system, yielding an average of 5.0 × 109 total nucleated cells from mobilized animals and 1.2 × 109 total nucleated cells from nonmobilized animals per procedure. This report provides sufficient detail to adapt this apheresis technique at other institutions, which will facilitate more efficient and detailed analysis of HSCs and their progeny blood cells.
Keyphrases
- hiv aids
- induced apoptosis
- antiretroviral therapy
- stem cells
- cell cycle arrest
- hematopoietic stem cell
- human immunodeficiency virus
- infectious diseases
- peripheral blood
- hiv infected
- cord blood
- endothelial cells
- cell therapy
- randomized controlled trial
- hiv positive
- signaling pathway
- case control
- optical coherence tomography
- oxidative stress
- mesenchymal stem cells
- minimally invasive
- combination therapy
- pi k akt
- cell proliferation
- molecular dynamics