Ethyl Acetate Fraction from Hedyotis diffusa plus Scutellaria barbata Suppresses Migration of Bone-Metastatic Breast Cancer Cells via OPN-FAK/ERK/NF-κB Axis.
Ting FangYing-Xuan YanYue YangYa-Xin LvQing-Qi ChangDan-Dan ZhangPublished in: Evidence-based complementary and alternative medicine : eCAM (2020)
Hedyotis diffusa plus Scutellaria barbata is a couplet of medicinal that has been commonly used to treat inflammation-related diseases and various types of tumors. However, the effect of this couplet on tumor cell migration has not been elucidated. With the aid of MCF-7-BOM, a bone-metastatic subline of ER + breast cancer MCF-7, we showed that ethyl acetate fraction extracted at an equal weight ratio of Hedyotis diffusa plus Scutellaria barbata (EA11) inhibited cell migration of MCF-7-BOM in a concentration-dependent manner. To define the underlying molecular mechanism, we revealed that EA11 reduced the expression of osteopontin (OPN) and interfered with the FAK/ERK/NF-κB signaling pathways, which are both critical for breast cancer bone metastasis. This study strongly suggested EA11 may represent a potential therapeutic agent against bone metastasis of breast cancer.
Keyphrases
- cell migration
- breast cancer cells
- signaling pathway
- pi k akt
- bone mineral density
- bone loss
- soft tissue
- squamous cell carcinoma
- small cell lung cancer
- oxidative stress
- bone regeneration
- epithelial mesenchymal transition
- induced apoptosis
- cell proliferation
- poor prognosis
- postmenopausal women
- body mass index
- ionic liquid
- body composition
- single cell
- endoplasmic reticulum stress
- weight loss
- inflammatory response
- weight gain
- endoplasmic reticulum