Cell type-specific cytonuclear coevolution in three allopolyploid plant species.
Keren ZhangXueru ZhaoYue ZhaoZhi-Bin ZhangZhijian LiuZiyu LiuYanan YuJuzuo LiYiqiao MaYuefan DongXi PangXin JinNing LiBao LiuJonathan F WendelJixian ZhaiYanping LongTianya WangLei GongPublished in: Proceedings of the National Academy of Sciences of the United States of America (2023)
Cytonuclear disruption may accompany allopolyploid evolution as a consequence of the merger of different nuclear genomes in a cellular environment having only one set of progenitor organellar genomes. One path to reconcile potential cytonuclear mismatch is biased expression for maternal gene duplicates (homoeologs) encoding proteins that target to plastids and/or mitochondria. Assessment of this transcriptional form of cytonuclear coevolution at the level of individual cells or cell types remains unexplored. Using single-cell (sc-) and single-nucleus (sn-) RNAseq data from eight tissues in three allopolyploid species, we characterized cell type-specific variations of cytonuclear coevolutionary homoeologous expression and demonstrated the temporal dynamics of expression patterns across development stages during cotton fiber development. Our results provide unique insights into transcriptional cytonuclear coevolution in plant allopolyploids at the single-cell level.
Keyphrases
- single cell
- poor prognosis
- rna seq
- gene expression
- transcription factor
- high throughput
- induced apoptosis
- machine learning
- cell death
- electronic health record
- bone marrow
- dna methylation
- genome wide
- mesenchymal stem cells
- deep learning
- risk assessment
- heat shock
- cell proliferation
- birth weight
- reactive oxygen species
- pregnant women
- cell wall