Low-cell-number, single-tube amplification (STA) of total RNA revealed transcriptome changes from pluripotency to endothelium.

Yi-Hsuan LeeYa-Wen HsuehYao-Hung PengKung-Chao ChangKuen-Jer TsaiH Sunny SunIh-Jen SuPo-Ming Chiang

Published in: BMC biology (2017)

Overall, STA offered a simple and sensitive way to concurrently quantify both mRNA and ncRNA expression in low-cell-number samples for both qPCR and high-throughput sequencing.

Keyphrases

single cell
rna seq
high throughput sequencing
cell therapy
poor prognosis
nitric oxide
gene expression
nucleic acid
stem cells
bone marrow
mesenchymal stem cells
label free