Collaborative study of thresholds for mutagens: proposal of a typical protocol for detection of hormetic responses in cytotoxicity tests.
Shizuyo SutouAkiko KoedaKana KomatsuToshiyuki ShiragikuHiroshi SekiKohji YamakageTakeru NiitsumaToshiyuki KudoAkihiro Wakatanull nullPublished in: Genes and environment : the official journal of the Japanese Environmental Mutagen Society (2018)
Hormetic responses in cytotoxicity tests were observed and thresholds could be established. Based on the results of this investigation, we put forward a tentative protocol to detect chemical hormesis in cytotoxicity tests, i.e., inoculate 2000 cells per well, add various doses of a test chemical 48 h after inoculation, add a detection dye 10 h after treatment, and measure optical density 2 h after addition of the reagent for detection.