Evaluation of sample preparation protocols for quantitative NMR-based metabolomics.

Olga A SnytnikovaAnastasiya A KhlichkinaRenad Z SagdeevYuri P Tsentalovich

Published in: Metabolomics : Official journal of the Metabolomic Society (2019)

The methanol-chloroform extraction seems to be an optimal method for tissues with the high lipid content, providing a satisfactory lipid removal and low metabolite losses. The ultrafiltration leads to large losses of metabolites (up to 60%) and for this reason is not suitable for quantitative analysis.

Keyphrases

high resolution
fatty acid
magnetic resonance
mass spectrometry
gene expression
ms ms
molecularly imprinted
carbon dioxide