CASP orter: A Novel Inducible Human CASP1/NALP3/ASC Inflammasome Biosensor.

Chan ZouJordan A BeardGuo-Ping YangWilliam E EvansErik J Bonten

Published in: Journal of inflammation research (2022)

We have established a cell-based CASP1/NLRP3 inflammasome model, utilizing a fluorescence biosensor as readout for qualitatively observing and quantitatively determining the activation of caspase 1 and NLRP3 inflammasomes in living cells and easily define the inhibitory effect of inhibitors with high efficacy.

Keyphrases

nlrp inflammasome
living cells
single molecule
fluorescent probe
sensitive detection
gold nanoparticles
endothelial cells
label free
quantum dots
single cell
cell death
cell therapy
induced pluripotent stem cells
pluripotent stem cells
induced apoptosis
stem cells