Angie-LAMP for diagnosis of human eosinophilic meningitis using dog as proxy: A LAMP assay for Angiostrongylus cantonensis DNA in cerebrospinal fluid.

Vojtech BalážPhoebe RivoryDouglas HaywardSusan JaenschRichard MalikRogan LeeDavid ModrýJan Šlapeta

Published in: PLoS neglected tropical diseases (2023)

The Angie-LAMP assay is a useful molecular tool for detecting Angiostrongylus DNA in canine CSF and performs comparably to a laboratory Angiostrongylus qPCR. Adaptation of single-step sample lysis improved potential applicability for diagnosis of angiostrongyliasis in a clinical setting for dogs and by extension, to humans.

Keyphrases

cerebrospinal fluid
loop mediated isothermal amplification
single molecule
circulating tumor
high throughput
cell free
endothelial cells
nucleic acid
sensitive detection
induced pluripotent stem cells
risk assessment
pluripotent stem cells
circulating tumor cells