RadA , a Key Gene of the Circadian Rhythm of Escherichia coli .
Aissatou Bailo DialloSoraya MezouarAsma BoumazaOksana FiammingoBenjamin CoiffardPierre PontarottiBenoit DesnuesJean-Louis MegePublished in: International journal of molecular sciences (2022)
Circadian rhythms are present in almost all living organisms, and their activity relies on molecular clocks. In prokaryotes, a functional molecular clock has been defined only in cyanobacteria. Here, we investigated the presence of circadian rhythms in non-cyanobacterial prokaryotes. The bioinformatic approach was used to identify a homologue of KaiC (circadian gene in cyanobacteria) in Escherichia coli . Then, strains of E. coli (wild type and mutants) were grown on blood agar, and sampling was made every 3 h for 24 h at constant conditions. Gene expression was determined by qRT-PCR, and the rhythmicity was analyzed using the Cosinor model. We identified RadA as a KaiC homologue in E. coli. Expression of radA showed a circadian rhythm persisting at least 3 days, with a peak in the morning. The circadian expression of other E. coli genes was also observed. Gene circadian oscillations were lost in radA mutants of E. coli . This study provides evidence of molecular clock gene expression in E. coli with a circadian rhythm. Such a finding paves the way for new perspectives in antibacterial treatment.
Keyphrases
- escherichia coli
- gene expression
- genome wide
- wild type
- dna methylation
- poor prognosis
- genome wide identification
- atrial fibrillation
- heart rate
- biofilm formation
- pseudomonas aeruginosa
- staphylococcus aureus
- transcription factor
- cystic fibrosis
- working memory
- anti inflammatory
- smoking cessation
- atomic force microscopy
- candida albicans
- silver nanoparticles
- real time pcr