Ribozyme-mediated CRISPR/Cas9 gene editing in pyrethrum (Tanacetum cinerariifolium) hairy roots using a RNA polymerase II-dependent promoter.
Jia-Wen LiTuo ZengZhi-Zhuo XuJin-Jin LiHao HuQin YuLi ZhouRi-Ru ZhengJing LuoCai-Yun WangPublished in: Plant methods (2022)
An A. rhizogenes-mediated hairy root transformation and ribozyme-mediated CRISPR/Cas9 gene editing system in pyrethrum was established, thereby facilitating gene editing in specific organs or at a particular developmental stage in future pyrethrum research.