A rapid and low-cost method for genomic DNA extraction from the cyanobacterium Synechocystis.
Dale J A HarrisonElinor P ThompsonPublished in: Biology methods & protocols (2020)
A two-step method is reported for preparation of genomic DNA from the model cyanobacterium Synechocystis that can be performed with minimal equipment and reagents in about an hour. High yields of genetic material can be obtained (200-450 ng/μl) with reasonable purity. A further ethanol precipitation step can be included but is not necessary if template is simply required for polymerase chain reaction (PCR) or digestion. This new protocol is helpful for amplification of genes of interest in early-stage research projects and for low throughput screening of transformants. It is more reliable than colony PCR of Synechocystis cultures, and less involved and cheaper than existing clean-DNA preparation methods. It represents an unusually simple and reliable extraction protocol for the growing body of research making use of this cyanobacterium.
Keyphrases
- circulating tumor
- low cost
- early stage
- cell free
- nucleic acid
- single molecule
- copy number
- molecularly imprinted
- randomized controlled trial
- genome wide
- blood pressure
- circulating tumor cells
- squamous cell carcinoma
- quality improvement
- real time pcr
- dna methylation
- high resolution
- transcription factor
- quantum dots
- neoadjuvant chemotherapy
- liquid chromatography
- anaerobic digestion