[Study of the sorptiondesorption of ecdysterone (20 E) as part of adaptogenic compositions with inulin and functional food ingredients based on spinach and quinoa].

The main principle in the enrichment of food with minor bioactive compounds is the prediction and evaluation of possible chemical interactions of the components included in the matrix of the food. These interactions have a impact on the bioavailability of minor bioactive compounds. In our work, we studied the processes of sorption and desorption (release), the main processes affecting the bioavailability of the minor bioactive compound ecdysterone (20 E) in the composition of functional food ingredients obtained from spinach leaves (FFI-1) and quinoa grains (FFI-2) on hydrocolloid matrix - inulin. The objective of the research was to study the completeness of sorption-desorption processes of 20 E in adaptogenic compositions with inulin and functional food ingredients based on spinach and quinoa under the influence of hydrolytic enzymes of the gastrointestinal tract (GIT) in vitro. Material and methods . To obtain experimental compositions, containing FFI-1 and FFI-2 and the polysaccharide (inulin), a mechanical mixing method was used. To study the sorption properties, model solutions of the compositions were prepared. Using an in vitro enzymatic model, the ability of 20 E to be released from the matrix of the compositions was studied. The content of 20 E was determined by HPLC-MS/MS. Results . 6 compositions with different ratios of polysaccharide/FFI were obtained. At the first stage of the study, the maximum sorption of 20E in the model solution was observed for 4 compositions with the ratio of inulin : FFI = 2.50 or 3.75 g : 189.19 mg FFI-1 or 68.40 mg FFI-2. At the second stage of the study, when assessing the desorption of 20 E on the enzymatic GIT model, it was found that 20 E almost completely released only from 2 compositions, in other cases about 25% of 20 E remained in a bound state. Conclusion . The formulation of two compositions with the ratio of inulin (2.50 g) : FFI-1 (189.19 mg)/FFI-2 (68.40 mg) were obtained, which have the most optimal sorption / release parameters of 20 E under the influence of human gastrointestinal enzymes. These compositions can be considered promising for inclusion in the formulation of fortified foods.