Determination of Gold Ions in Human Urine Using Genetically Engineered Microorganisms on a Paper Device.
Kai-Hong GuoPei-Hsuan ChenChieh LinChien-Fu ChenI-Ren LeeYi-Chun YehPublished in: ACS sensors (2018)
This paper presents a whole-cell biosensor that operates in conjunction with a smartphone-based fluorescence diagnostic system on a paper device to monitor the concentration of gold ions in human urine. The heavy metal-tolerant bacteria Cupriavidus metallidurans was genetically engineered for use as a chassis in a red fluorescent protein (RFP)-based microbial sensor. The biosensor is highly sensitive to gold ions, with a detection limit of 110 nM. The proposed smartphone-based analysis system provides a user-friendly approach to design tools of personal health monitoring for reporting the presence of gold ions in human urine.
Keyphrases
- quantum dots
- endothelial cells
- label free
- heavy metals
- induced pluripotent stem cells
- pluripotent stem cells
- gold nanoparticles
- healthcare
- sensitive detection
- stem cells
- emergency department
- mental health
- aqueous solution
- mesenchymal stem cells
- photodynamic therapy
- cell therapy
- water soluble
- binding protein
- amino acid
- small molecule
- mass spectrometry
- single molecule