Development of a whole-cell biosensor for β-lactamase inhibitor discovery.
Mitchell A JeffsRachel A V GrayPrameet M ShethChristopher T LohansPublished in: Chemical communications (Cambridge, England) (2023)
The production of β-lactamases by bacterial pathogens endangers antimicrobial therapy, and new inhibitors for β-lactamases are urgently needed. We report the development of a luminescent-based biosensor that quantifies β-lactamase inhibition in a cellular context, based on the activation of transcriptional factor AmpR following the exposure of bacterial cells to β-lactams. This rapid method can account for factors like membrane permeability and can be employed to identify new β-lactamase inhibitors.
Keyphrases
- gram negative
- escherichia coli
- klebsiella pneumoniae
- sensitive detection
- multidrug resistant
- quantum dots
- gold nanoparticles
- induced apoptosis
- label free
- cell therapy
- single cell
- small molecule
- cell cycle arrest
- stem cells
- high throughput
- antimicrobial resistance
- cell death
- endoplasmic reticulum stress
- metal organic framework