Targeted RNAseq assay incorporating unique molecular identifiers for improved quantification of gene expression signatures and transcribed mutation fraction in fixed tumor samples.

Chunxiao FuMichal MarczykMichael SamuelsAlexander J TrevartonJiaxin QuRosanna LauLili DuTodd PappasBruno V SinnRebekah E GouldLajos PusztaiChristos HatzisWilliam Fraser Symmans

Published in: BMC cancer (2021)

Modifications to the targeted RNAseq protocol for SET4 assay significantly increased the precision of UMI-based and reads-based measurements of individual transcripts, multi-gene signatures, and mutant transcript fraction, particularly with FFPE samples.

Keyphrases

gene expression
genome wide
high throughput
dna methylation
cancer therapy
randomized controlled trial
copy number
rna seq