Biocompatible Lipid Polymer Cationic Nanoparticles for Antigen Presentation.
Yunys Pérez-BetancourtBianca de Carvalho Lins Fernandes TávoraEliana L Faquim-MauroAna Maria Carmona-RibeiroPublished in: Polymers (2021)
Biocompatible lipid polymer nanoparticles (NPs) previously used as antimicrobial agents are explored here as immuno-adjuvants. Poly (methyl methacrylate) (PMMA)/dioctadecyldimethylammonium bromide (DODAB)/poly (diallyldimethylammonium chloride) (PDDA) nanoparticles (NPs) were prepared by emulsion polymerization of methyl methacrylate (MMA) in the presence of DODAB and PDDA, with azobisisobutyronitrile (AIBN) as the initiator. NPs characterization after dialysis by dynamic light-scattering yielded 225 ± 2 nm hydrodynamic diameter (Dz), 73 ± 1 mV zeta-potential (ζ), and 0.10 ± 0.01 polydispersity (P). Ovalbumin (OVA) adsorption reduced ζ to 45 ± 2 mV. Balb/c mice immunized with NPs/OVA produced enhanced OVA-specific IgG1 and IgG2a, exhibited moderate delayed type hypersensitivity reaction, and enhanced cytokines production (IL-4, IL-10, IL-2, IFN-γ) by cultured spleen cells. There was no cytotoxicity against cultured macrophages and fibroblasts. Advantages of the PMMA/DODAB/PDDA NPs were high biocompatibility, zeta-potential, colloidal stability, and antigen adsorption. Both humoral and cellular antigen-specific immune responses were obtained.
Keyphrases
- immune response
- oxide nanoparticles
- endothelial cells
- induced apoptosis
- dendritic cells
- chronic kidney disease
- staphylococcus aureus
- cell cycle arrest
- fatty acid
- aqueous solution
- type diabetes
- end stage renal disease
- human health
- walled carbon nanotubes
- toll like receptor
- risk assessment
- oxidative stress
- drug induced
- peritoneal dialysis
- skeletal muscle
- endoplasmic reticulum stress
- wild type