An Improved Method for Assessing Antigen Presentation on the Surface of Plasmodium falciparum-Infected Erythrocytes by Immuno-Electron Microscopy.
Marek CyrklaffCecilia Palmira SanchezLukas HanebutteJulia JägerUlrich Sebastian SchwarzMichael LanzerPublished in: Methods in molecular biology (Clifton, N.J.) (2022)
Immuno-electron microscopy can detect and localize antigens in cells or tissues at a resolution of several nanometers. In the case of P. falciparum-infected erythrocytes, immuno-EM studies are frequently hampered by the electron-dense nature of the hemoglobin and access of antibodies to antigenic sites, particularly if the targeted protein is presented on the host cell surface or lies in proximity to the host cell cytoskeleton. Here, we describe an improved immuno-EM protocol that overcomes these problems. The improved signal to noise ratio and the enhanced access to antigenic sites now allows one to obtain information regarding target density and distribution and, hence, additional insights into the architecture and function of parasite-induced, or -affected, structures.
Keyphrases
- electron microscopy
- plasmodium falciparum
- cell surface
- induced apoptosis
- randomized controlled trial
- mental health
- gene expression
- single cell
- high resolution
- cell cycle arrest
- healthcare
- stem cells
- cancer therapy
- dendritic cells
- health information
- cell death
- binding protein
- cell proliferation
- mass spectrometry
- amino acid
- case report
- toxoplasma gondii