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Application and Mechanism of Cryptolepine and Neocryptolepine Derivatives as T3SS Inhibitors for Control of Bacterial Leaf Blight on Rice.

Jiang ShaoZhi-Jun ZhangYu ShiWei-Qi JiangFaisal SiddiqueLiangye ChenGenyan LiuJia-Kai ZhuXiong-Fei LuoYing-Qian LiuJun-Xia AnCheng-Jie YangZi-Ning Cui
Published in: Journal of agricultural and food chemistry (2024)
Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv oryzae ( Xoo ) is extremely harmful to rice production. The traditional control approach is to use bactericides that target key bacterial growth factors, but the selection pressure on the pathogen makes resistant strains the dominant bacterial strains, leading to a decline in bactericidal efficacy. Type III secretion system (T3SS) is a conserved and critical virulence factor in most Gram-negative bacteria, and its expression or absence does not affect bacterial growth, rendering it an ideal target for creating drugs against Gram-negative pathogens. In this work, we synthesized a range of derivatives from cryptolepine and neocryptolepine. We found that compound Z-8 could inhibit the expression of Xoo T3SS-related genes without affecting the growth of bacteria. an in vivo bioassay showed that compound Z-8 could effectively reduce the hypersensitive response (HR) induced by Xoo in tobacco and reduce the pathogenicity of Xoo in rice. Furthermore, it exhibited synergy in control of bacterial leaf blight when combined with the quorum quenching bacterial F20.
Keyphrases
  • gram negative
  • escherichia coli
  • poor prognosis
  • multidrug resistant
  • staphylococcus aureus
  • pseudomonas aeruginosa
  • type iii
  • biofilm formation
  • binding protein
  • antimicrobial resistance