Simultaneous and multiplexed phenotyping of circulating exosomes with the orthogonal CRISPR-Cas platform.

Gaoxing SuMengting XuYuedong ZhuYan ZhangYanan LinYanyan Yu

Published in: Chemical communications (Cambridge, England) (2024)

Simultaneous and multiplexed exosome protein profiling via an orthogonal CRISPR-Cas platform was achieved in this work. Aptamers were recruited to translate exosome surface protein information into Cas12a/Cas13a cleavage activity. The established multiplexed platform performed robustly with biological matrixes and could profile exosome proteins in clinical serum samples.

Keyphrases

crispr cas
genome editing
high throughput
single cell
protein protein
stem cells
mesenchymal stem cells
amino acid
binding protein