Integrated analysis of long non-coding RNAs and mRNAs associated with malignant transformation of gastrointestinal stromal tumors.
Xiaonan YinYuan YinLei DaiChaoyong ShenNa ChenJunshu LiZhaolun CaiZhiyuan JiangJian WangZhou ZhaoXin ChenHongxin DengBo ZhangPublished in: Cell death & disease (2021)
Malignant transformation of gastrointestinal stromal tumors (GISTs) is correlated with poor prognosis; however, the underlying biological mechanism is not well understood. In the present study, low-risk (LR) GISTs, GISTs categorized as high-risk based on tumor size (HBS), and on mitotic rate (HBM) were collected for RNA sequencing. Candidate hub lncRNAs were selected by Oncomine analysis. Expression of a selected hub lncRNA, DNM3OS, and its correlation with patients' prognosis were analyzed using FISH staining, followed with the determination of function and underlying mechanism. Our results revealed a series of key pathways and hub lncRNAs involved in the malignant transformation of GISTs. Oncomine analysis revealed a tight association between clinical signatures and DNM3OS and suggested that DNM3OS is a hub lncRNA that is involved in the Hippo signaling pathway. In addition, DNM3OS was upregulated in HBS, HBM, and HBS/M GIST and correlated with worse prognosis in patients with GISTs. In addition, DNM3OS promoted GIST cell proliferation and mitosis by regulating the expression of GLUT4 and CD36. Collectively, these results improve our understanding of the malignant transformation of GISTs and unveil a series of hub lncRNAs in GISTs.
Keyphrases
- poor prognosis
- long non coding rna
- network analysis
- cell proliferation
- signaling pathway
- bioinformatics analysis
- single cell
- end stage renal disease
- chronic kidney disease
- ejection fraction
- cell cycle
- newly diagnosed
- epithelial mesenchymal transition
- gene expression
- peritoneal dialysis
- genome wide analysis
- genome wide
- oxidative stress
- binding protein
- dna methylation
- endoplasmic reticulum stress
- patient reported outcomes