An Outbreak of Newcastle Disease Virus in the Moscow Region in the Summer of 2022.
Artyom RtishchevAnastasia TreshchalinaElena ShustovaElizaveta BoravlevaAlexandra GambaryanPublished in: Veterinary sciences (2023)
In August 2022 on a backyard farm in the Moscow region of Russia, mortality was observed among chickens, and all 45 birds of a particular farm died or were slaughtered after the onset of symptoms within a few days. Paramyxovirus was isolated from the diseased birds. Based on the nucleotide sequences of the F and NP gene fragments, it was determined that the virus belonged to subgenotype VII.1 AAvV-1 class II. The cleavage site of the F gene 109 SGGRRQKRFIG 119 and T in 546 and 555 position of the NP gene were typical for the velogenic type. The genetically closest NDV isolates were found in Iran. The mean time of death of 10-day-old chicken embryos upon infection with the minimal infectious dose was 52 h, which is typical for the velogenic pathotype. The virus caused 100% death of six-week-old chickens during oral infection as well as 100% mortality of all contact chickens, including those located in remote cages, which proves the ability of the virus to spread not only by the fecal-oral route but also by the aerosol route. That demonstrates a high level of pathogenicity and contagiousness of the isolated strain for chicken. However, mice intranasally infected with high doses of the virus did not die.
Keyphrases
- disease virus
- copy number
- genome wide
- cardiovascular events
- risk factors
- heat stress
- randomized controlled trial
- cardiovascular disease
- clinical trial
- escherichia coli
- physical activity
- type diabetes
- staphylococcus aureus
- depressive symptoms
- transcription factor
- genetic diversity
- genome wide analysis
- pseudomonas aeruginosa