Resveratrol, a stilbene, particularly trans-isomer, shows significant osteogenic potential but experiences high instability and poor bioavailability. However, cis-isomer (cRes) is not explored yet due to its instability. Our study investigates the osteoinductive potential of cRes for the first time by stabilizing it onto the surface of gold nanoparticles. cRes capped GNPs (cRGNPs) presented no toxic effects on the MC3T3-E1 cells with increased levels of alkaline phosphatase and calcium deposition. The nanoparticles presented a 2.6-fold increase in cell number compared to the control. The pro-migratory effect of the cRGNPs was also significantly higher (97.21 ± 0.99 % migration) in 4 days. The osteoinductivity was further confirmed by enhanced expression of osteoblastic genes like RUNX2, OPN, OCN, BMP, OPG, and Col1A. The stability provided to cRes upon conjugating to GNPs allowed exploration of its potential in aiding proliferation, migration, and differentiation of the pre-osteoblasts, which will be beneficial in repairing bone defects.
Keyphrases
- gold nanoparticles
- mesenchymal stem cells
- induced apoptosis
- human health
- poor prognosis
- signaling pathway
- mental health
- single cell
- reduced graphene oxide
- bone marrow
- risk assessment
- gene expression
- cell therapy
- stem cells
- cell death
- cell cycle arrest
- genome wide
- dna methylation
- vascular smooth muscle cells
- bone regeneration
- climate change
- binding protein
- walled carbon nanotubes