A Highly Sensitive CRISPR-Empowered Surface Plasmon Resonance Sensor for Diagnosis of Inherited Diseases with Femtomolar-Level Real-Time Quantification.
Fei ZhengZhi ChenJingfeng LiRui WuBin ZhangGuohui NieZhongjian XieQizhen ZhangPublished in: Advanced science (Weinheim, Baden-Wurttemberg, Germany) (2022)
The clustered regularly interspaced short palindromic repeats (CRISPR) molecular system has emerged as a promising technology for the detection of nucleic acids. Herein, the development of a surface plasmon resonance (SPR) sensor that is functionalized with a layer of locally grown graphdiyne film, achieving excellent sensing performance when coupled with catalytically deactivated CRISPR-associated protein 9 (dCas9), is reported. dCas9 protein is immobilized on the sensor surface and complexed with a specific single-guide RNA, enabling the amplification-free detection of target sequences within genomic DNA. The sensor, termed CRISPR-SPR-Chip, is used to successfully analyze recombinant plasmids with only three-base mutations with a limit of detection as low as 1.3 fM. Real-time monitoring CRISPR-SPR-Chip is used to analyze clinical samples of patients with Duchenne muscular dystrophy with two exon deletions, which are detected without any pre-amplification step, yielding significantly positive results within 5 min. The ability of this novel CRISPR-empowered SPR (CRISPR-eSPR) sensing platform to rapidly, precisely, sensitively, and specifically detect a target gene sequence provides a new on-chip optic approach for clinical gene analysis.
Keyphrases
- genome wide
- genome editing
- crispr cas
- dna methylation
- copy number
- duchenne muscular dystrophy
- label free
- high throughput
- nucleic acid
- loop mediated isothermal amplification
- gene expression
- escherichia coli
- single molecule
- optical coherence tomography
- multidrug resistant
- small molecule
- quantum dots
- mass spectrometry
- ionic liquid
- single cell
- living cells
- liquid chromatography
- fluorescent probe