Improving AOX1 promoter efficiency by overexpression of Mit1 transcription factor.
Samin Haghighi PoodehSeyed Omid Ranaei SiadatSareh ArjmandMaryam Khalifeh SoltaniPublished in: Molecular biology reports (2022)
Overexpression of Mit1 could be considered a promising strategy for upregulation of target recombinant proteins in Pichia pastoris. Intracellular overexpression of Mit1 upregulates the heterologous target gene (eGFP) production, which is expressed under the control of pAOX1.