Purification of the Dynamin-Related Protein Vps1 Using Mammalian and Bacterial Expression Systems.
Natalia V VarlakhanovaMarijn G J FordPublished in: Methods in molecular biology (Clifton, N.J.) (2021)
The dynamin-related proteins (DRPs) are self-assembling membrane remodeling machines that are indispensable for fundamental cellular trafficking and homeostatic processes. We describe in this chapter protocols developed in our laboratory for purification of full-length and minimal constructs of Chaetomium thermophilum Vps1, the model fungal DRP, using mammalian and Escherichia coli expression systems.