Electrospun Membrane for the Extraction of Acrylamide in Pet Food Samples.

A simple microextraction procedure was developed using an electrospun nanostructured membrane to determine acrylamide in pet food samples. Polyvinyl chloride, polyvinyl alcohol, and polyvinyl alcohol/hydroxyethyl cellulose electrospun membranes were prepared and investigated as a sorbent to extract acrylamide. The characterization of the synthesized electrospun membrane was accomplished using field-emission scanning electron microscopy (FESEM). FESEM images showed uniform morphology and beadless nanofibers. Quantification was done by high-performance liquid chromatography with ultraviolet detection. A series of microextraction parameters were optimized before quantitative analysis of dry pet food samples. The calibration curve exhibited good linearity with a correlation coefficient of 0.996 across a 1-100 μg/kg concentration range. The recovery of acrylamide for pet food samples spiked with 5 and 10 μg/kg was in the range of 79.6-113.9 (n = 3). The intraday precision of the method was less than 12% for three replicated real spiked samples at the 5 μg/kg level. The results demonstrated that the electrospun nanostructured membrane has good extraction selectivity and minimal matrix effect with an enrichment factor of 180-fold.