Chemoproteomics reveals Sofalcone inhibits the inflammatory response of Caco-2 cells by covalently targeting HMGB1.
Tong YangDandan LiuYulei LiYing ZhangYinhua ZhuJunzhe ZhangChen WangShujie ZhangYin Kwan WongPiao LuoQiuyan GuoFei XiaTianyu ZhongHuan TangJi-Gang WangPublished in: Chemical communications (Cambridge, England) (2023)
Sofalcone (Sof), a synthetic analog of sophoradin, is a type of natural phenol derived from the traditional medicinal herb Sophora subprostrata , with potent anti-inflammatory activity. However, the mechanisms of action of Sof for treating intestinal-associated inflammation are not well known. In this work, we identified high mobility group box 1 (HMGB1) as the key covalent target of Sof for the anti-inflammatory activity in the human colonic epithelial cells through quantitative chemoproteomics profiling.
Keyphrases
- inflammatory response
- induced apoptosis
- endothelial cells
- oxidative stress
- cell cycle arrest
- transcription factor
- lipopolysaccharide induced
- induced pluripotent stem cells
- high resolution
- single cell
- cancer therapy
- pluripotent stem cells
- endoplasmic reticulum stress
- lps induced
- binding protein
- signaling pathway
- anti inflammatory
- mass spectrometry
- pi k akt
- immune response