In Vivo Two-Photon Microscopy Reveals Sensory-Evoked Serotonin (5-HT) Release in Adult Mammalian Neocortex.
Gabriel Ocana-SanteroAdam M PackerTrevor SharpSimon J B ButtPublished in: ACS chemical neuroscience (2024)
The recent development of genetically encoded fluorescent neurotransmitter biosensors has opened the door to recording serotonin (5-hydroxytryptamine, 5-HT) signaling dynamics with high temporal and spatial resolution in vivo . While this represents a significant step forward for serotonin research, the utility of available 5-HT biosensors remains to be fully established under diverse in vivo conditions. Here, we used two-photon microscopy in awake mice to examine the effectiveness of specific 5-HT biosensors for monitoring 5-HT dynamics in somatosensory cortex. Initial experiments found that whisker stimulation evoked a striking change in 5-HT biosensor signal. However, similar changes were observed in controls expressing green fluorescent protein, suggesting a potential hemodynamic artifact. Subsequent use of a second control fluorophore with emission peaks separated from the 5-HT biosensor revealed a reproducible, stimulus-locked increase in 5-HT signal. Our data highlight the promise of 5-HT biosensors for in vivo application, provided measurements are carried out with appropriate optical controls.
Keyphrases
- label free
- quantum dots
- living cells
- single molecule
- high resolution
- type diabetes
- gold nanoparticles
- randomized controlled trial
- high speed
- high throughput
- machine learning
- sensitive detection
- computed tomography
- electronic health record
- metabolic syndrome
- magnetic resonance imaging
- single cell
- fluorescent probe
- small molecule
- binding protein
- insulin resistance
- optical coherence tomography
- mass spectrometry
- climate change