Inductively coupled plasma mass spectrometry method for plasma and intracellular antimony quantification applied to pharmacokinetics of meglumine antimoniate.
Diana J Garay-BaqueroDavid E Rebellón-SánchezMiguel D PrietoLina Giraldo-ParraAdriana NavasSheryl AtkinsonStuart McDougallMaría Adelaida GómezPublished in: Bioanalysis (2021)
Background: A high-throughput method using inductively coupled plasma mass spectrometry (ICP-MS) was developed and validated for the quantitative analysis of antimony in human plasma and peripheral blood mononuclear cells from patients with cutaneous leishmaniasis undergoing treatment with meglumine antimoniate. Materials & methods: Antimony was digested in clinical samples with 1% tetramethylammonium hydroxide/1% EDTA and indium was used as internal standard. Accuracy, precision and stability were evaluated. Conclusion: Taking the lower limit of quantitation to be the lowest validation concentration with precision and accuracy within 20%, the current assay was successfully validated from 25 to 10000 ng/ml for antimony in human plasma and peripheral blood mononuclear cells. This protocol will serve as a baseline for future analytical designs, aiming to provide a reference method to allow inter-study comparisons.
Keyphrases
- mass spectrometry
- liquid chromatography
- high performance liquid chromatography
- high throughput
- capillary electrophoresis
- high resolution
- gas chromatography
- ms ms
- tandem mass spectrometry
- randomized controlled trial
- simultaneous determination
- multiple sclerosis
- current status
- liquid chromatography tandem mass spectrometry
- gold nanoparticles
- single cell
- sewage sludge
- case control