SEUSS integrates transcriptional and epigenetic control of root stem cell organizer specification.
Huawei ZhaiXiaoyue ZhangYanrong YouLihao LinWenkun ZhouChuan-You LiPublished in: The EMBO journal (2020)
Proper regulation of homeotic gene expression is critical for stem cell fate in both plants and animals. In Arabidopsis thaliana, the WUSCHEL (WUS)-RELATED HOMEOBOX 5 (WOX5) gene is specifically expressed in a group of root stem cell organizer cells called the quiescent center (QC) and plays a central role in QC specification. Here, we report that the SEUSS (SEU) protein, homologous to the animal LIM-domain binding (LDB) proteins, assembles a functional transcriptional complex that regulates WOX5 expression and QC specification. SEU is physically recruited to the WOX5 promoter by the master transcription factor SCARECROW. Subsequently, SEU physically recruits the SET domain methyltransferase SDG4 to the WOX5 promoter, thus activating WOX5 expression. Thus, analogous to its animal counterparts, SEU acts as a multi-adaptor protein that integrates the actions of genetic and epigenetic regulators into a concerted transcriptional program to control root stem cell organizer specification.
Keyphrases
- cell fate
- gene expression
- stem cells
- transcription factor
- dna methylation
- binding protein
- arabidopsis thaliana
- genome wide
- poor prognosis
- dna binding
- genome wide identification
- induced apoptosis
- copy number
- protein protein
- signaling pathway
- cell therapy
- dna damage
- cell cycle arrest
- dna repair
- amino acid
- quality improvement
- mesenchymal stem cells
- heat shock
- small molecule
- endoplasmic reticulum stress