Lactate entrance into the brain facilities adipose tissue lipolysis during exercise via circulating calcitonin gene-related peptide.
Malihe AvesehMaryam Koushkie-JahromiJavad NematiSaeed Esmaeili-MahaniNajmeh Sadat HosseiniPublished in: Archives of physiology and biochemistry (2023)
Objectives : We assessed the relationships between CGRP, lactate and fat regulation. Methods : We evaluated the effect of intracerebroventricular (i.c.v.) injection of lactate and acute exercise on brain CGRP expression, and its concentration in serum/cerebrospinal fluid (SCF) in rats. Results : Injection of lactate up-regulated CGRP expression in the cortex and CSF and activated p38-mitogen-activated protein kinases (p38-MAPK) pathway. Co-injection of lactate and sb203580, deterred lactate-induced up-regulation of CGRP in the brain and CSF. Exercise increased the CGRP expression in the brain and CSF and up-regulated fat metabolism. Inhibition of lactate entrance into the brain using alpha-cyano-4-hydroxycinnamate (4-CIN) diminished exercise-induced CGRP up-regulation in the brain and CSF. Reducing the circulating blood lactate by pre-treatment of the animals with dichloroacetate (DCA) had no effect on exercise-induced increase in CGRP expression or fat metabolism during exercise. Conclusions : lactate probably acts as one of a signalling molecule in the brain to regulate fat metabolism during exercise.
Keyphrases
- adipose tissue
- resting state
- white matter
- functional connectivity
- poor prognosis
- high intensity
- cerebrospinal fluid
- physical activity
- cerebral ischemia
- multiple sclerosis
- high fat diet
- transcription factor
- type diabetes
- oxidative stress
- ultrasound guided
- smoking cessation
- high glucose
- body composition
- acute respiratory distress syndrome