Identification of Potential Auxin-Responsive Small Signaling Peptides through a Peptidomics Approach in Arabidopsis thaliana.
Weigui LuoYuan XiaoQiwen LiangYi SuLang-Tao XiaoPublished in: Molecules (Basel, Switzerland) (2019)
Small signaling peptides (SSPs) are a class of short peptides playing critical roles in plant growth and development. SSPs are also involved in the phytohormone signaling pathway. However, identification of mature SSPs is still a technical challenge because of their extremely low concentrations in plant tissue and complicated interference by many other metabolites. Here, we report an optimized protocol to extract SSPs based on protoplast extraction and to analyze SSPs based on tandem mass spectrometry peptidomics. Using plant protoplasts as the material, soluble peptides were directly extracted into phosphate buffer. The interference of non-signaling peptides was significantly decreased. Moreover, we applied the protocol to identify potential SSPs in auxin treated wild type and auxin biosynthesis defective mutant yuc2yuc6. Over 100 potential SSPs showed a response to auxin in Arabidopsis thaliana.
Keyphrases
- arabidopsis thaliana
- wild type
- tandem mass spectrometry
- plant growth
- amino acid
- signaling pathway
- randomized controlled trial
- ultra high performance liquid chromatography
- simultaneous determination
- ms ms
- epithelial mesenchymal transition
- oxidative stress
- cell wall
- high resolution
- induced apoptosis
- risk assessment
- bioinformatics analysis
- climate change
- drug delivery
- mass spectrometry