Photoprotective Mechanism of Fucoxanthin in Ultraviolet B Irradiation-Induced Retinal Müller Cells Based on Lipidomics Analysis.
Yixin ShiJiaying RenBaomin ZhaoTaihai ZhuHang QiPublished in: Journal of agricultural and food chemistry (2022)
Long-term exposure to sunlight and/or blue light causes vision damage to people of all ages. Dietary pigments and polyphenols have been shown to have photoprotective potential for eyes; however, many unknowns regarding the protective mechanism remain. In this study, we used ultraviolet B (UVB) irradiation-induced retinal Müller cells (RMCs) to screen for dietary polyphenols and pigment compounds with effective photoprotective activity. Fucoxanthin (FX) was shown to have the best therapeutic effect, and the mechanism was evaluated via lipidomics analysis. Both intra- and extracellular ROS, mitochondrial depolarization, and DNA damage induced by UVB irradiation were inhibited by FX. Meanwhile, FX modulated the MAPK signaling pathway, which is correlated with apoptosis and inflammation. Our lipidomics data revealed that FX regulated lipid metabolism disorder and protected the membrane structure. These results confirm the effective photoprotective effects of FX, which may lead to new insights into FX-functionalized photoprotective foods.
Keyphrases
- oxidative stress
- induced apoptosis
- diabetic rats
- cell cycle arrest
- dna damage
- signaling pathway
- pi k akt
- optical coherence tomography
- cell death
- endoplasmic reticulum stress
- diabetic retinopathy
- transcription factor
- drug induced
- radiation induced
- epithelial mesenchymal transition
- quantum dots
- machine learning
- single cell
- dna repair
- reactive oxygen species
- mass spectrometry
- light emitting
- molecularly imprinted