Maintenance and differentiation of human ES cells on polyvinylidene fluoride scaffolds immobilized with a vitronectin-derived peptide.
Sang Eun ParkGyu-Bum YeonHui-Gwan GooDong Sik SeoAhmed A DayemKyung Eun LeeHyun-Mee ParkSsang-Goo ChoDae-Sung KimPublished in: Journal of cellular physiology (2020)
Polyvinylidene fluoride (PVDF) is biocompatible, easy to fabricate, and has piezoelectric properties; it has been used for many biomedical applications including stem cell engineering. However, long-term cultivation of human embryonic stem cells (hESCs) and their differentiation toward cardiac lineages on PVDF have not been investigated. Herein, PVDF nanoscaled membrane scaffolds were fabricated by electrospinning; a vitronectin-derived peptide-mussel adhesive protein fusion (VNm) was immobilized on the scaffolds. hESCs cultured on the VNm-coated PVDF scaffold (VNm-PVDF scaffold) were stably expanded for more than 10 passages while maintaining the expression of pluripotency markers and genomic integrity. Under cardiac differentiation conditions, hESCs on the VNm-PVDF scaffold generated more spontaneously beating colonies and showed the upregulation of cardiac-related genes, compared with those cultured on Matrigel and VNm alone. Thus, VNm-PVDF scaffolds may be suitable for the long-term culture of hESCs and their differentiation into cardiac cells, thus expanding their application in regenerative medicine.
Keyphrases
- tissue engineering
- endothelial cells
- induced apoptosis
- left ventricular
- embryonic stem cells
- stem cells
- poor prognosis
- cell cycle arrest
- ionic liquid
- endoplasmic reticulum stress
- cell death
- gene expression
- drug delivery
- binding protein
- long non coding rna
- bone marrow
- protein protein
- high resolution
- atomic force microscopy
- cell therapy
- drug release