Molecular Requirements for Self-Interaction of the Respiratory Syncytial Virus Matrix Protein in Living Mammalian Cells.
Marta TrevisanVeronica Di AntonioAnnalisa RadeghieriGiorgio PalùReena GhildyalGualtiero AlvisiPublished in: Viruses (2018)
Respiratory syncytial virus (RSV) is an important human pathogen, which infects respiratory tract epithelial cells causing bronchiolitis and pneumonia in children and the elderly. Recent studies have linked RSV matrix (M) ability to self-interaction and viral budding. However, RSV M has been crystalized both as a monomer and a dimer, and no formal proof exists to date that it forms dimers in cells. Here, by using a combination of confocal laser scanning microscopy and bioluminescent resonant energy transfer applied to differently tagged deletion mutants of RSV M, we show that the protein can self-interact in living mammalian cells and that both the N and C-terminus of the protein are strictly required for the process, consistent with the reported dimeric crystal structure.
Keyphrases
- respiratory syncytial virus
- respiratory tract
- energy transfer
- crystal structure
- protein protein
- high resolution
- endothelial cells
- binding protein
- induced apoptosis
- amino acid
- single molecule
- young adults
- optical coherence tomography
- cell cycle arrest
- high speed
- intensive care unit
- small molecule
- signaling pathway
- case control
- extracorporeal membrane oxygenation
- cell death
- pluripotent stem cells
- mechanical ventilation
- pi k akt