Human and murine fibroblast single cell transcriptomics reveals fibroblast clusters are differentially affected by ageing, and serum cholesterol.
Kim van KuijkIan R McCrackenRenée J H A TillieS E J AsselberghsDlzar A KhederS MuitjensHan JinR S TaylorR Wichers SchreurChristoph KuppeRoss DobiePrakash RamachandranMarion J J GijbelsLieve TemmermanPhoebe M KirkwoodJ LuytenYanming LiHeidi NoelsPieter G GoossensJohn R Wilson-KanamoriLeon J SchurgersY H ShenBarend M E MeesErik A L BiessenNeil Cowan HendersonRafael KramannAndrew H BakerJudith C SluimerPublished in: Cardiovascular research (2023)
We provide two adventitial fibroblast-specific markers, PDGFRA and DPEP1, and demonstrate fibroblast heterogeneity in health and cardiovascular disease in humans and mice. Biological relevance is evident from regulation of fibroblast clusters by age and hypercholesterolemia in vivo, associations with human atherosclerotic plaque traits, and enrichment of genes with a GWAS for CVD.
Keyphrases
- single cell
- endothelial cells
- cardiovascular disease
- rna seq
- public health
- wound healing
- genome wide
- healthcare
- mental health
- type diabetes
- induced pluripotent stem cells
- coronary artery disease
- high throughput
- dna methylation
- risk assessment
- metabolic syndrome
- insulin resistance
- skeletal muscle
- high fat diet induced
- climate change
- human health