Evaluation of factors influencing expression and extraction of recombinant bacteriophage endolysins in Escherichia coli.
Cecilia Lucía BalabanCristian Alejandro SuárezCarina Andrea BoncompainNatalia Peressutti-BacciEduardo Augusto CeccarelliHéctor Ricardo MorbidoniPublished in: Microbial cell factories (2022)
-tag location and lysis buffer additives (e.g. N-lauroylsarcosine), are sensible strategies to obtain higher levels of soluble S. aureus endolysins.