Real-Time Imaging of Bacteria/Osteoblast Dynamic Coculture on Bone Implant Material in an in Vitro Postoperative Contamination Model.
Victor PrévostKarine AnselmeOlivier GalletMathilde HindiéTatiana PetithoryJules ValentinMathieu VeuilletLydie PlouxPublished in: ACS biomaterials science & engineering (2019)
Biomedical implants are an important part of evolving modern medicine but have a potential drawback in the form of postoperative pathogenic infection. Accordingly, the "race for surface" combat between invasive bacteria and host cells determines the fate of implants. Hence, proper in vitro systems are required to assess effective strategies to avoid infection. In this study, we developed a real time observation model, mimicking postoperative contamination, designed to follow E. coli proliferation on a titanium surface occupied by human osteoblastic progenitor cells (STRO). This model allowed us to monitor E. coli invasion of human cells on titanium surfaces coated and uncoated with fibronectin. We showed that the surface colonization of bacteria is significantly enhanced on fibronectin coated surfaces irrespective of whether areas were uncovered or covered with human cells. We further revealed that bacterial colonization of the titanium surfaces is enhanced in coculture with STRO cells. Finally, this coculture system provides a comprehensive system to describe in vitro and in situ bacterial and human cells and their localization but also to target biological mechanisms involved in adhesion as well as in interactions with surfaces, thanks to fluorescent labeling. This system is thus an efficient method for studies related to the design and function of new biomaterials.
Keyphrases
- biofilm formation
- induced apoptosis
- patients undergoing
- escherichia coli
- cell cycle arrest
- soft tissue
- risk assessment
- signaling pathway
- endothelial cells
- pseudomonas aeruginosa
- bone regeneration
- cell death
- staphylococcus aureus
- cell migration
- health risk
- bone mineral density
- quantum dots
- cystic fibrosis
- body composition
- pi k akt
- photodynamic therapy
- pluripotent stem cells