Electrochemical nano-biosensor based on electrospun indium zinc oxide nanofibers for the determination of complement component 3 protein.
Dinesh Ramkrushna RotakeTanmoya Nemai GhoshShiv Govind SinghPublished in: Mikrochimica acta (2023)
Age-related macular degeneration (AMD) is a progressive chronic neurodegenerative retinal disease leading to vision loss, irreversible blindness, and visual impairment in older adults worldwide. Complement component 3 (C3) protein has been identified as the most predominant biomarker towards early diagnosis of AMD; therefore, there is an utmost requirement for non-invasive detection of C3 protein in the tear fluids of AMD patients. Considering this, we report an insightful electrochemical sensor capable of detecting clinically relevant concentrations ranging from 10 fg/mL to 1 μg/mL using electrospun indium-doped zinc oxide (InZnO) nanofibers as the transducing layer. The InZnO nanofibers have facilitated high anti-C3 antibody loading of 3.42 × 10 -9 mol/cm 2 and enhanced the overall charge transport mechanism at the sensor interface. The biofunctionalization process of the biosensor was investigated thoroughly using X-ray photoelectron spectroscopy (XPS) as well as different electrochemical techniques. The target C3 proteins were captured on the fabricated biosensor surface and determined through changes in charge transfer resistance (R CT ) while executing electrochemical impedance spectroscopy (EIS) and peak current (I p ) in the case of cyclic voltammetry (CV) and differential pulse voltammetry (DPV), respectively. The InZnO nanofiber-based nano-biosensor demonstrated a very low limit of detections (LODs) of 5.214 fg/mL and 0.241 fg/mL with an excellent sensitivity of 4.6709 (ΔR/R) (g/mL) -1 cm -2 and 54.4939 (ΔI p /I p )% (g/mL) -1 cm -2 for EIS and DPV techniques, respectively. By virtue of high antibody loading, ultrasensitive and ultra-selective capability, the indium-doped ZnO nanofibers show huge potential to be used as a high-performance diagnostic platform for AMD diagnosis.
Keyphrases
- label free
- age related macular degeneration
- gold nanoparticles
- quantum dots
- high resolution
- molecularly imprinted
- sensitive detection
- end stage renal disease
- dual energy
- reduced graphene oxide
- protein protein
- multiple sclerosis
- ionic liquid
- newly diagnosed
- binding protein
- peritoneal dialysis
- computed tomography
- single molecule
- chronic kidney disease
- oxide nanoparticles
- magnetic resonance imaging
- prognostic factors
- small molecule
- physical activity
- tissue engineering
- high throughput
- visible light
- magnetic resonance
- solid state
- metal organic framework
- wound healing
- lactic acid