A sensitive luminescence syncytium induction assay (LuSIA) based on a reporter plasmid containing a mutation in the glucocorticoid response element in the long terminal repeat U3 region of bovine leukemia virus.

Hirotaka SatoSonoko WatanukiLanlan BaiLiushiqi BorjiginHiroshi IshizakiYasunobu MatsumotoYuma HachiyaHiroshi SentsuiYoko Aida

Published in: Virology journal (2019)

The new LuSIA protocol is quantitative and more sensitive than the previous assay based on CC81-BLU3G cells and should facilitate development of several new BLV assays.

Keyphrases

high throughput
induced apoptosis
crispr cas
cell cycle arrest
escherichia coli
randomized controlled trial
acute myeloid leukemia
quantum dots
bone marrow
endoplasmic reticulum stress
cell death
single cell
oxidative stress
light emitting