Biological and phylogenetic characteristics of West African lineages of West Nile virus.
Gamou FallNicholas Di PaolaMartin FayeMoussa DiaCaio César de Melo FreireCheikh LoucoubarPaolo Marinho de Andrade ZanottoOusmane FayeAmadou Alpha SallPublished in: PLoS neglected tropical diseases (2017)
The West Nile virus (WNV), isolated in 1937, is an arbovirus (arthropod-borne virus) that infects thousands of people each year. Despite its burden on global health, little is known about the virus' biological and evolutionary dynamics. As several lineages are endemic in West Africa, we obtained the complete polyprotein sequence from three isolates from the early 1990s, each representing a different lineage. We then investigated differences in growth behavior and pathogenicity for four distinct West African lineages in arthropod (Ap61) and primate (Vero) cell lines, and in mice. We found that genetic differences, as well as viral-host interactions, could play a role in the biological properties in different WNV isolates in vitro, such as: (i) genome replication, (ii) protein translation, (iii) particle release, and (iv) virulence. Our findings demonstrate the endemic diversity of West African WNV strains and support future investigations into (i) the nature of WNV emergence, (ii) neurological tropism, and (iii) host adaptation.
Keyphrases
- global health
- escherichia coli
- genome wide
- staphylococcus aureus
- public health
- biofilm formation
- sars cov
- transcription factor
- amino acid
- gene expression
- disease virus
- small molecule
- skeletal muscle
- copy number
- mass spectrometry
- insulin resistance
- high fat diet induced
- brain injury
- protein protein
- aedes aegypti
- single molecule
- high speed