DNA double-strand breaks in human induced pluripotent stem cell reprogramming and long-term in vitro culturing.
Pavel ŠimaraLenka TesarovaDaniela RehakovaPavel MatulaStanislav StejskalAles HamplIrena KoutnaPublished in: Stem cell research & therapy (2017)
Our observations suggest that cellular reprogramming increases the DSB number but that the repair mechanism functions well. However, after prolonged in vitro culturing of hiPSCs, the repair capacity decreases.