DNA double-strand breaks in human induced pluripotent stem cell reprogramming and long-term in vitro culturing.

Pavel ŠimaraLenka TesarovaDaniela RehakovaPavel MatulaStanislav StejskalAles HamplIrena Koutna

Published in: Stem cell research & therapy (2017)

Our observations suggest that cellular reprogramming increases the DSB number but that the repair mechanism functions well. However, after prolonged in vitro culturing of hiPSCs, the repair capacity decreases.

Keyphrases

stem cells
endothelial cells
high glucose
diabetic rats
circulating tumor
cell free
induced pluripotent stem cells
single molecule
drug induced
pluripotent stem cells
bone marrow
circulating tumor cells