Immunogenic SARS-CoV2 Epitopes Defined by Mass Spectrometry.
Ke PanYulun ChiuEric HuangMichelle ChenJunmei WangIvy LaiShailbala SinghRebecca ShawMichael J MacCossCassian YeePublished in: bioRxiv : the preprint server for biology (2021)
Current state of the art uses putative epitope peptides based on in silico prediction algorithms to evaluate the T cell response among COVID-19 patients. However, none of these peptides have been tested for immunogenicity, i.e. the ability to elicit a T cell response capable of recognizing endogenously presented peptide. In this study, we used MHC immune-precipitation, acid elution and tandem mass spectrometry to define the SARS-CoV-2 immunopeptidome for membrane glycol-protein and the non-structural protein. Furthermore, taking advantage of a highly robust endogenous T cell (ETC) workflow, we verify the immunogenicity of these MS-defined peptides by in vitro generation of MGP and NSP13 peptide-specific T cells and confirm T cell recognition of MGP or NSP13 endogenously expressing cell lines.
Keyphrases
- sars cov
- mass spectrometry
- tandem mass spectrometry
- liquid chromatography
- amino acid
- high performance liquid chromatography
- gas chromatography
- ultra high performance liquid chromatography
- respiratory syndrome coronavirus
- high resolution mass spectrometry
- simultaneous determination
- machine learning
- high resolution
- multiple sclerosis
- protein protein
- ms ms
- molecular docking
- binding protein
- electronic health record
- molecular dynamics simulations
- coronavirus disease