mus-52 disruption and metabolic regulation in Neurospora crassa: Transcriptional responses to extracellular phosphate availability.
Maíra Pompeu MartinsEriston V GomesPablo R SanchesWellington R PedersoliNilce Maria Martinez-RossiAntonio RossiPublished in: PloS one (2018)
Advances in the understanding of molecular systems depend on specific tools like the disruption of genes to produce strains with the desired characteristics. The disruption of any mutagen sensitive (mus) genes in the model fungus Neurospora crassa, i.e. mus-51, mus-52, or mus-53, orthologous to the human genes KU70, KU80, and LIG4, respectively, provides efficient tools for gene targeting. Accordingly, we used RNA-sequencing and reverse transcription-quantitative polymerase chain reaction amplification techniques to evaluate the effects of mus-52 deletion in N. crassa gene transcriptional modulation, and thus, infer its influence regarding metabolic response to extracellular availability of inorganic phosphate (Pi). Notably, the absence of MUS-52 affected the transcription of a vast number of genes, highlighting the expression of those coding for transcription factors, kinases, circadian clocks, oxi-reduction balance, and membrane- and nucleolus-related proteins. These findings may provide insights toward the KU molecular mechanisms, which have been related to telomere maintenance, apoptosis, DNA replication, and gene transcription regulation, as well as associated human conditions including immune system disorders, cancer, and aging.
Keyphrases
- genome wide identification
- transcription factor
- genome wide
- genome wide analysis
- endothelial cells
- dna binding
- copy number
- dna methylation
- induced pluripotent stem cells
- gene expression
- escherichia coli
- bioinformatics analysis
- pluripotent stem cells
- endoplasmic reticulum stress
- papillary thyroid
- drug delivery
- binding protein
- drug induced
- nucleic acid