Disparate pathways for extrachromosomal DNA biogenesis and genomic DNA repair.
John C RoseIvy Tsz-Lo WongBence DanielMatthew G JonesKathryn E YostKing L HungEllis J CurtisPaul S MischelHoward Y ChangPublished in: bioRxiv : the preprint server for biology (2023)
Our study harnesses a CRISPR-based method to examine ecDNA biogenesis, uncovering efficient circularization between DSBs. ecDNAs and their corresponding chromosomal scars can form via NHEJ or MMEJ, but the ecDNA and scar formation processes are distinct. Based on our findings, we establish a mechanistic model of excisional ecDNA formation.