Harnessing the intrinsic photochemistry of isoxazoles for the development of chemoproteomic crosslinking methods.
Marshall G LougeeVinayak Vishnu PagarHee Jong KimSamantha X PancoeW Kit ChiaRobert H MachBenjamin A GarciaE James PeterssonPublished in: Chemical communications (Cambridge, England) (2022)
The intrinsic photochemistry of the isoxazole, a common heterocycle in medicinal chemistry, can be applied to offer an alternative to existing strategies using more perturbing, extrinsic photo-crosslinkers. The utility of isoxazole photo-crosslinking is demonstrated in a wide range of biologically relevant experiments, including common proteomics workflows.