Direct evidence of host-mediated glycosylation of NleA and its dependence on interaction with the COPII complex.
Lindsay BurnsFrançois Le MauffSamantha GruenheidPublished in: Gut microbes (2024)
Non-LEE-encoded Effector A (NleA) is a type III secreted effector protein of enterohaemorrhagic and enteropathogenic Escherichia coli as well as the related mouse pathogen Citrobacter rodentium . NleA translocation into host cells is essential for virulence. We previously published several lines of evidence indicating that NleA is modified by host-mediated mucin-type O-linked glycosylation, the first example of a bacterial effector protein modified in this way. In this study, we use lectins to provide direct evidence for the modification of NleA by O-linked glycosylation and determine that the interaction of NleA with the COPII complex is necessary for this modification to occur.
Keyphrases
- type iii
- escherichia coli
- regulatory t cells
- induced apoptosis
- dendritic cells
- biofilm formation
- pseudomonas aeruginosa
- staphylococcus aureus
- randomized controlled trial
- systematic review
- candida albicans
- binding protein
- immune response
- cell cycle arrest
- cystic fibrosis
- antimicrobial resistance
- signaling pathway
- small molecule
- oxidative stress
- pi k akt
- multidrug resistant