Self-Assembled Nanoconstructs Modified with Amplified Aptamers Inhibited Tumor Growth and Retinal Vascular Hyperpermeability via Vascular Endothelial Growth Factor Capturing.
Jihyun LeeByung Joo LeeYeong Mi LeeHansoo ParkJeong Hun KimWon Jong KimPublished in: Molecular pharmaceutics (2017)
Here, nanoconstructs consisting of a DNA-amplified aptamer with a biocompatible polymer backbone for capturing target biomolecules are presented. First, the polymer-DNA nanoconstructs were prepared by hybridization of two complementary single-stranded DNAs that were each conjugated to a dextran polymer backbone. The designed polymer-DNA amplified aptamer nanoconstructs (PA-aNCs) were then prepared by utilizing polymer-DNA nanoconstructs conjugated with an aptamer (PA-NCs) using a rolling circle amplification reaction to amplify the aptamer. These PA-aNCs were successfully applied to alleviate tumor growth and vascular endothelial growth factor (VEGF)-induced retinal vascular hyperpermeability in vivo through the highly effective capture of human VEGF as a target molecule. These PA-aNCs could be used as therapeutic agent for anti-VEGF therapy by efficiently capturing human VEGF.
Keyphrases
- vascular endothelial growth factor
- endothelial cells
- nucleic acid
- high glucose
- gold nanoparticles
- circulating tumor
- single molecule
- sensitive detection
- label free
- cell free
- magnetic nanoparticles
- optical coherence tomography
- photodynamic therapy
- pluripotent stem cells
- induced pluripotent stem cells
- optic nerve
- mesenchymal stem cells
- drug delivery
- ionic liquid
- replacement therapy
- cell therapy
- electron transfer