Analysis of Methylesterase Gene Family in Fragaria vesca Unveils Novel Insights into the Role of FvMES2 in Methyl Salicylate-Mediated Resistance against Strawberry Gray Mold.
Ruimin JiaKeyan XingLin TianXiaomin DongLigang YuXihui ShenYang WangPublished in: Journal of agricultural and food chemistry (2024)
Methylesterases (MESs) hydrolyze carboxylic ester and are important for plant metabolism and defense. However, the understanding of MES' role in strawberries against pathogens remains limited. This study identified 15 FvMESs with a conserved catalytic triad from the Fragaria vesca genome. Spatiotemporal expression data demonstrated the upregulated expression of FvMESs in roots and developing fruits, suggesting growth involvement. The FvMES promoter regions harbored numerous stress-related cis -acting elements and transcription factors associated with plant defense mechanisms. Moreover, FvMES 2 exhibited a significant response to Botrytis cinerea stress and showed a remarkable correlation with the salicylic acid (SA) signaling pathway. Molecular docking showed an efficient binding potential between FvMES2 and methyl salicylate (MeSA). The role of FvMES2 in MeSA demethylation to produce SA was further confirmed through in vitro and in vivo assays. After MeSA was applied, the transient overexpression of FvMES 2 in strawberries enhanced their resistance to B. cinerea compared to wild-type plants.
Keyphrases
- molecular docking
- transcription factor
- poor prognosis
- wild type
- signaling pathway
- binding protein
- molecular dynamics simulations
- dna methylation
- epithelial mesenchymal transition
- long non coding rna
- dna binding
- stress induced
- high throughput
- cell proliferation
- electronic health record
- pi k akt
- risk assessment
- genome wide
- gram negative
- human health
- climate change
- endoplasmic reticulum stress
- data analysis
- plant growth
- water quality