Polypyrrole-palladium nanocomposite as a high-efficiency transducer for thrombin detection with liposomes as a label.
Ajay Kumar YagatiArne BehrentVanessa TomanekSachin Ganpat ChavanAnna GoSung Ryul ParkZhengzhi JinAntje J BaeumnerTaesung KimPublished in: Analytical and bioanalytical chemistry (2021)
Sensitive and selective determination of protein biomarkers with high accuracy often remains a great challenge due to their existence in the human body at an exceptionally low concentration level. Therefore, sensing mechanisms that are easy to use, simple, and capable of accurate quantification of analyte are still in development to detect biomarkers at a low concentration level. To meet this end, we demonstrated a methodology to detect thrombin in serum at low concentration levels using polypyrrole (PPy)-palladium (Pd)nanoparticle-based hybrid transducers using liposomes encapsulated redox marker as a label. The morphology of Ppy-Pd composites was characterized by scanning electron microscopy, and the hybrid structure provided excellent binding and detection platform for thrombin detection in both buffer and serum solutions. For quantitative measurement of thrombin in PBS and serum, the change in current was monitored using differential pulse voltammetry, and the calculated limit of quantification (LOQ) and limit of detection (LOD) for the linear segment (0.1-1000 nM of thrombin) were 1.1 pM and 0.3 pM, in serum, respectively. The sensors also exhibited good stability and excellent selectivity towards the detection of thrombin, and thus make it a strong candidate for adopting its sensing applications in biomarker detection technologies.
Keyphrases
- loop mediated isothermal amplification
- real time pcr
- reduced graphene oxide
- label free
- drug delivery
- high resolution
- high efficiency
- high throughput
- air pollution
- blood pressure
- particulate matter
- gold nanoparticles
- heavy metals
- mass spectrometry
- binding protein
- molecularly imprinted
- risk assessment
- solid phase extraction
- pluripotent stem cells